Expression and glycosylation studies of human FGF receptor 4
H Tuominen, P Heikinheimo, BM Loo, K Kataja… - Protein Expression and …, 2001 - Elsevier
Fibroblast growth factor receptor subtype 4 (FGFR4) has been shown to have special
activation properties and just one splicing form, unlike the other FGFRs. FGFR4
overexpression is correlated with breast cancer and therefore FGFR4 is a target for drug
design. Our aim is to overexpress high amounts of homogeneous FGFR4 extracellular
domain (FGFR4ed) for structural studies. We show that baculovirus-insect cell-expressed
FGFR4ed is glycosylated on three (N88, N234, and N266) of the six possible N …
activation properties and just one splicing form, unlike the other FGFRs. FGFR4
overexpression is correlated with breast cancer and therefore FGFR4 is a target for drug
design. Our aim is to overexpress high amounts of homogeneous FGFR4 extracellular
domain (FGFR4ed) for structural studies. We show that baculovirus-insect cell-expressed
FGFR4ed is glycosylated on three (N88, N234, and N266) of the six possible N …
