Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues
MJ Churchill, PR Gorry, D Cowley, L Lal… - Journal of …, 2006 - Taylor & Francis
Journal of neurovirology, 2006•Taylor & Francis
The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1)
in the brain remains elusive. By combining immunohistochemistry, laser capture
microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes
and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects.
The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent
reservoir of provirus in brain that will impact the development and likely success of strategies …
in the brain remains elusive. By combining immunohistochemistry, laser capture
microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes
and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects.
The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent
reservoir of provirus in brain that will impact the development and likely success of strategies …
The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.
Taylor & Francis Online