[HTML][HTML] Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver
L Sokol, V Geldhof, M García-Caballero, NV Conchinha… - STAR protocols, 2021 - Elsevier
STAR protocols, 2021•Elsevier
Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct
phenotypes and functional adaptations that are dependent on their physiological
environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to
low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their
functions. Here, we describe protocols combining magnetic and fluorescent activated cell
sorting for rapid and reproducible EC purification from four adult murine tissues. For …
phenotypes and functional adaptations that are dependent on their physiological
environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to
low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their
functions. Here, we describe protocols combining magnetic and fluorescent activated cell
sorting for rapid and reproducible EC purification from four adult murine tissues. For …
Summary
Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their functions. Here, we describe protocols combining magnetic and fluorescent activated cell sorting for rapid and reproducible EC purification from four adult murine tissues.
For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020).
Elsevier